Seminar: Real-Time Near Infrared Fluorescence Imaging

Prof. Donal O’Shea

Head of the Department of Pharmaceutical and Medicinal Chemistry,
Royal College of Surgeons in Ireland

Real-Time Near Infrared Fluorescence Imaging – from chemistry to mice and the potential for beyond

Fluorescence imaging, utilizing molecular fluorophores, often acts as a central tool for the investigation of fundamental biological processes.  It also offers huge future potential for human imaging coupled to therapeutic procedures such as fluorescence guided surgery. We have recently developed a new class of near infrared (NIR) fluorophore from which excellent in vitro and in vivo imaging probes can be developed.1 But in spite of the advantages offered by longer wavelength NIR emissions a common limitation with fluorescence imaging is the difficulty in discriminating non-specific fluorescence from fluorescence localized at a specific region of interest. This can restrict imaging to individual time points at which non-specific background fluorescence has been minimized.  It would be of significant advantage if the fluorescence output could be modulated from off to on in response to specific biological events as this would permit imaging of such events in real time without background interference. Two approaches (one molecular and one nanoparticle based) to achieve this using cellular endocytosis as the NIR-fluorescence switching trigger will be described.2  Both approaches permit continuous real-time imaging of the cellular uptake, trafficking and efflux processes as extracellular fluorophore is non-fluorescent. The principles behind the NIR-fluorescence off/on switching will be explained and illustrated in vitro and in vivo. In addition, a theranostics approach using the combination of NIR fluorescence imaging and photodynamic therapy will also be shown.3

Please feel welcome to attend on the 6th of December 2013 at 9:10 am. The talk will be held in Cheyne Lecture Theatre at RCSI, St. Stephen’s Green.

1.         (a) Tasior, M.; O’Shea, D.F. Bioconjugate Chem. 2010, 21, 1130. (b) Wu Dan, O’Shea D.F. Org. Lett. 2013, 15, 3392.

2.         (a) Palma, A.; Alvarez, L.A.; Frimannsson, D.O.; Grossi, M.; Quinn, S.J.; O’Shea, D.F. J. Am. Chem. Soc. 2011, 133, 19618. (b) http://www.youtube.com/watch?v=FjipbGTf8w4.

3.         O’Connor, A. E.; McGee, M. M.; Likar, Y.; Ponomarev, V.; Callanan, J. J.; O’Shea, D. F.; Byrne, A. T.; Gallagher, W. M. Intern. J. Cancer, 2012, 130, 705.

 

Workshop: Light sheet fluorescence microscopy

Date: Monday 25th of November
Venue: Royal College of Surgeons in Ireland, 123 St Stephen’s Green, Dublin 2
Organisers: Dr Emmanuel G. Reynaud (UCD), Dr Heiko Dussmann (RCSI), Dr Chris Power (Zeiss)

Light sheet microscopy is an emerging technology that enables long term imaging of large biological specimen with minimal photo damage. It opens new avenues to study cell biological and developmental processes with unprecedented resolution in space and time and facilitates systems biology approaches by quantitative imaging of entire living systems. The life science research community is eager to use light sheet microscopy inspired by the many spectacular proof of principle papers, but is less aware of the technological and computational challenges associated with the light sheet microscopy paradigm.

Schedule: Monday 25th of November
Morning (9-12 am): Open to all

Introduction: Dr Emmanuel G. Reynaud (UCD), Dr Heiko Dussmann (RCSI)

  • Dr Bill Chaudhry, Newcastle University:Introduction to Light Sheet Microscopy
  • Dr Chris Power, Carl Zeiss UK: Zeiss Light Sheet Z-1
  • Ellen Baker, Hamamatsu: “Cameras fro Light Sheet Microscopy”
  • Dr Emmanuel G. Reynaud, University College Dublin: Challenges in Light Sheet Microscopy

Lunch-time:  3 quick introductions to the Zeiss Lightsheet Z-1

Afternoon (1pm- 5pm): three groups of 5 persons.

  • Session 1: Sample preparation, Dr Emmanuel G. Reynaud
  • Session 2: Light Sheet Z.1, Dr Chris Power and Dr Heiko Dussmann
  • Session 3: How to build a light sheet microscope, Dr Bill Chaudhry
  • Last session: Data management, Dr Chris Power and Dr Emmanuel G. Reynaud

Evening (5pm- 7pm): Discussion and Q&A

Registration:
Send application requests to microimaging@rcsi.ie with a short description of your research/needs for 3D microscopy.

Fees:
A small fee (20€) will apply to cover room rental and consumables. A 50% discount will apply for MSI and RMS members.

Further training options:
Demonstrations: Besides the workshop, there will be demo time slots available during the week after the 25th of November, if you are interested please contact Dr Chris Power (via email Chris.Power@zeiss.com) and include a brief description of your potential own sample you want to image.

Training sessions: We will run two additional training sessions depending on numbers and requests.
Date: 21st of November 1.30 pm-5 pm

  • Session: Cell Biology and Tissue Engineering (10 persons max.)
  • Dr Malte Wachsmuth, EMBL: “Observing single molecules with a light-sheet microscopeTalk free for all, more details here

Date: 2nd of December 1.30 pm-5 pm

  • Session: Extra class


Accommodation:

Hotels in close proximity: St. Stephen’s Green Hotel, Travelodge and Albany House
We might be able to arrange for corporate rates, please contact us for more information.

Low-Budget: Avalon House is a hostel just down the road from the conference venue. Rooms range from single bedrooms to large dorms. Prices start from 12 EUR; if it happens to be your birthday, you might get a bed for free!

Sponsors:

Download flyer here

Seminar: light sheet microscopy

Dr. Malte Wachsmuth

Cell Biology & Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany

Observing single molecules with a light-sheet microscope

As part of the workshop on light sheet fluorescence microscopy, the Centre for Systems Medicine is pleased to announce a seminar about this thriving imaging technique. Dr. Wachsmuth performed his PhD research at the Division Biophysics of Macromolecules, German Cancer Research Center (DKFZ) in 2002 and gained several years of expertise in imaging techniques working for Leica Microsystems, University of Heidelberg, and as group leader of the Cell Biophysics Group, Institut Pasteur Korea, Seoul, South Korea. Since 2007 he is scientist in the Cell Biology & Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg.

In his current research, Dr. Wachsmuth and his team focus on extending and integrating imaging modalities, building new instrumentation for visual biochemistry and the structure and dynamics of the cell nucleus.

More information can be found on his research homepage.

The talk is free and open to everyone and will be held in Newman Study, RCSI on the 21st of November 2013 at 2 pm.

More information about the workshop is available here.