Molecular and Cellular Imaging Core

The Molecular and Cellular Imaging Core funded by the NBIPI and SFI is overseen and operated by Heiko Düssmann, Dr rer nat, Director of Molecular Imaging and Brenton Cavanagh, PhD, Research Engineer. Access is regulated according to the NBIPI access policy.

Zeiss LSM 5 live Duoscan Laser Scanning Microscope. Purpose of this equipment: live cell imaging, FRET, fast 3D imaging, FRAP Information: INDIMO 1080, inverted microscope, small stage incubator, piezo focus, motorised stage, laser lines: 405, 489, 561 nm. External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €40 half day, €40 whole night. Service €90/hr. Incubation €0.12/hour, email hduessmann@rcsi.ie for support and booking information.

 

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Light Sheet Fluorescence Microscope (Lightsheet Z1).  The LSFM is suitable for live biological samples using a 20x 1.0 NA water immersion imaging lens and 2x 5x 0.1NA cylindrical lenses as well as optically cleared samples using a dedicated 20x immersion objective with 1.0NA and 2 x 10x 0.2NA Cylindrical lenses. Excitation at 405, 488, 561 and 638 nm is available. For booking and costs please contact Heiko Duessmann. For description of spectral properties of the imaging light path. please also contact Heiko Duessmann. 

see link below for two examples –

Using the clearing techniques: Nice video of a view into a hippocampus of tie-2-GFP expressing mouse. Clearing, imaging and image processing (FiJi, Blender, Fluorender) by Brenton Cavanagh using the Lightsheet Z1. 

https://twitter.com/CentreSysmed?ref_src=twsrc%5Etfw%7Ctwcamp%5Eembeddedtimeline%7Ctwterm%5Eprofile%3ACentreSysmed&ref_url=http%3A%2F%2Fwww.systemsmedicineireland.ie%2 

N14-Spheroid2-Hoechst-PI-Fusion Of 5 Dual ViewsDual label live cell imaging of Nuclei in a N14-1208 patient-derived glioblastoma neurosphere with Hoechst (cyan) and PI(red, stains only dead cells), sample preparation by Viktorija Juric, imaging and image processing (FiJi) by Heiko Düssmann using the Lightsheet Z1. 

https://twitter.com/CentreSysmed?ref_src=twsrc%5Etfw%7Ctwcamp%5Eembeddedtimeline%7Ctwterm%5Eprofile%3ACentreSysmed&ref_url=http%3A%2F%2Fwww.systemsmedicineireland.ie%2F 

LSM 710Zeiss LSM 710 Confocal Microscope.  Purpose of this equipment: live cell imaging, spectral imaging, FRET, 3D imaging, FRAP
Inverted microscope, small stage incubator, piezo stage insert, hardware autofocus, laser lines: 405, 458, 488, 514, 543, 561, 633 nm
External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €40 half day, €40 whole night. Service €90/hr. Incubation €0.12/hour, contact hduessmann@rcsi for support and bookings

Cancer_Cells_Alive

Nice example of a 3D reconstruction of live DU145 prostate cancer cells stained with Calcein (cytosol, dark blue), TMRM (functional mitochondria, red) and Hoechst (DNA, cyan), the left cell is dividing, acquired on LSM 710 by Heiko Düssmann. 

 

 

 

Zeiss Aviovert 200M, Automated Epi-fluorescence Microscope: Purpose of this equipment: Live cell imaging, FRET, Calcium imaging, Autofluorescence (NADH), multiple fields of view. Information: Inverted microscope, small stage incubator, excitation and emission filter wheels, motorised stage, back illuminated EM-CCD (Andor ixon DU-897), MetaMorph External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €20 half day, €20 whole night. Service €80/hr. Incubation €0.12/hour 
                                                                      Contact: Heiko Dussmann – email:  hdussmann@rcsi.ie 
                                                                            Location: RCSI York Street, Dublin 2, Ireland   

Zeiss Aviovert 200M, Automated Epi-fluorescence Microscope

Purpose of this equipment: Live cell imaging, FRET, multiple fields of view . Information: Inverted microscope, big incubator, motorised stage, monochromator for excitation, 2 emission filter wheels on dual camera adaptor (2x Hamamatsu Orca 285), MetaMorph . External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €20 half day, €20 whole night. Service €80/hr. Incubation €0.12/hour 
Contact: Heiko Dussmann – email:  hdussmann@rcsi.ie 
Location: RCSI York Street, Dublin 2, Ireland

 

 

AIS-2: Automated microinjection system on Zeiss Aviovert 200M Epi-fluorescence Microscope 
Purpose of this equipment: automated microinjection of multiple fields of view, live cell imaging, FRET. Information: inverted microscope, incubator, excitation and emission filter wheels, motorised stage, EM-CCD (Hamamatsu Orca 9100-02), AIS2 and Axiovision.  External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €20 half day, €20 whole night. Service €80/hr. Incubation €0.12/hour 
Contact: Heiko Dussmann – email:  hdussmann@rcsi.ie 
Location: RCSI York Street, Dublin 2, Ireland

cellomix arrayscan VTICellomics Arrayscan VTI (including incubator & liquid handling robot) Cellomics Arrayscan VTI (including incubator & liguid handling robot). Purpose of this equipment: fluorescence imaging of large populations of cells in culture. 
Information: inverted fluorescence wide-field microscope, dry lenses, motorized stage compatible with multiwell or slide formats, CO2 and temperature control, liquid handling system. 
External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €60 whole day. Service €90/hr. Incubation €0.12/hour 

Zeiss Cell discoverer 7: An automated boxed microscope system calibrates itself, detects and focuses on the samples while the optics adjust themselves.  This makes it a reliable high content screening live cell imaging platform. Person to contact for training and advice on the CD7: Dr Brenton Cavanagh, Research Engineer for Microscopy, Office of Research and Innovation: email  -brentoncavanagh@rcsi.ie for support and booking information

Hitachi H7650 Transmission Electron Microscope: A high resolution microscope capable of resolving objects as small as 2nm.  It allows the visualization of objects that are otherwise invisible to light microscopy. It is suitable for thin sections of biological sample, DNA origami and nanoparticle characterization. Person to contact for training and advice on the TEM: Dr Brenton Cavanagh, Research Engineer for Microscopy, Office of Research and Innovation: email  -brentoncavanagh@rcsi.ie for support and booking information.