Molecular and Cellular Imaging Core

Molecular and Cellular Imaging Core

The Molecular and Cellular Imaging Core funded by the NBIPI and SFI is overseen and operated by Dr Heiko Duessmann, Director of Molecular Imaging. Access is regulated according to the NBIPI access policy.

  • Zeiss LSM 5 live Duoscan Laser Scanning Microscope
LSM 5live

Zeiss LSM 5 live Duoscan Laser Scanning Microscope

Purpose of this equipment: live cell imaging, FRET, fast 3D imaging, FRAP
Information: INDIMO 1080, inverted microscope, small stage incubator, piezo focus, motorised stage, laser lines: 405, 489, 561 nm. External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €40 half day, €40 whole night. Service €90/hr. Incubation €0.12/hour

 

  • Light Sheet Fluorescence Microscope (Lightsheet Z1)

IMG_2205 (002)

Instrument description: 

Instrument description: The LSFM is suitable for live biological samples using a 20x 1.0 NA water immersion imaging lens and 2x 5x 0.1NA cylindrical lenses as well as optically cleared samples using a dedicated 20x immersion objective with 1.0NA and 2 x 10x 0.2NA Cylindrical lenses. Excitation at 405, 488, 561 and 638 nm is available. For booking and costs please contact Heiko Duessmann. For description of spectral properties of the imaging light path please also contact Heiko Duessmann.

  • Zeiss LSM 710 Confocal Microscope
LSM 710

Zeiss LSM 710 Confocal Microscope

Purpose of this equipment: live cell imaging, spectral imaging, FRET, 3D imaging, FRAP
Information: inverted microscope, small stage incubator, piezo stage insert, hardware autofocus, laser lines: 405, 458, 488, 514, 543, 561, 633 nm
External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €40 half day, €40 whole night. Service €90/hr. Incubation €0.12/hour

Cancer_Cells_Alive

 

Nice example of a 3D reconstruction of live DU145 prostate cancer cells stained with Calcein (cytosol, dark blue), TMRM (functional mitochondria, red) and Hoechst (DNA, cyan), the left cell is dividing, acquired on LSM 710 by Heiko Düssmann.

Zeiss Aviovert 200M, Automated Epi-fluorescence Microscope

Purpose of this equipment: Live cell imaging, FRET, Calcium imaging, Autofluorescence (NADH), multiple fields of view.

Information: Inverted microscope, small stage incubator, excitation and emission filter wheels, motorised stage, back illuminated EM-CCD (Andor ixon DU-897), MetaMorph

External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €20 half day, €20 whole night. Service €80/hr. Incubation €0.12/hour
Contact: Heiko Dussmann – email:  hdussmann@rcsi.ie
Location: RCSI York Street, Dublin 2, Ireland   

Nikon TE2000, Automated Epi-fluorescence Microscope

Purpose of this equipment: Live cell imaging, FRET, multiple fields of view

Information: Inverted microscope, big incubator, motorised stage, monochromator for excitation, 2 emission filter wheels on dual camera adaptor (2x Hamamatsu Orca 285), MetaMorph

External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €20 half day, €20 whole night. Service €80/hr. Incubation €0.12/hour
Contact: Heiko Dussmann – email:  hdussmann@rcsi.ie
Location: RCSI York Street, Dublin 2, Ireland   

AIS-2: Automated microinjection system on Zeiss Aviovert 200M Epi-fluorescence Microscope
Purpose of this equipment: automated microinjection of multiple fields of view, live cell imaging, FRET

Information: inverted microscope, incubator, excitation and emission filter wheels, motorised stage, EM-CCD (Hamamatsu Orca 9100-02), AIS2 and Axiovision

External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €20 half day, €20 whole night. Service €80/hr. Incubation €0.12/hour
Contact: Heiko Dussmann – email:  hdussmann@rcsi.ie
Location: RCSI York Street, Dublin 2, Ireland   

 

 

 

 

 

 

Cellomics Arrayscan VTI (including incubator & liquid handling robot)

cellomix arrayscan VTI

Cellomics Arrayscan VTI (including incubator & liguid handling robot)

Purpose of this equipment: fluorescence imaging of large populations of cells in culture.
Information: inverted fluorescence wide-field microscope, dry lenses, motorized stage compatible with multiwell or slide formats, CO2 and temperature control, liquid handling system.
External users costs: Training/Supervision – €60/hr. Unsupervised sessions – €60 whole day. Service €90/hr. Incubation €0.12/hour

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